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			FINAL REPORT OF LABORATORY EXAMINATION Research Animal Diagnostic Laboratory 4011 Discovery Drive, Columbia MO 65201 1-800-669-0825 1-573-882-5983 radil@missouri.edu http://www.radil.missouri.edu/  | 
		
| CASE NUMBER: 9931-2009 | RECEIVED ON: 4/7/2009 | 
| COMPLETED ON: 4/15/2009 | 
SUBMITTED BY:
  Masahito Ikawa
  Osaka University
  Research Institute for Microbial Diseases
  3-1 Yamadaoka
  Suita 5650871
  Japan
  81-6-6879-8338 
SPECIMEN DESCRIPTION:
  SPECIES:  mouse
  DESCRIPTION:  ES cells
  NUMBER OF SPECIMENS:  2
  DATE SAMPLE(S) COLLECTED:  4/6/2009
  USE OF ANIMAL:  n/a
| ID | Investigator | Strain | Sex | ID on Tube | 
| 01 | ikawa | EGR-05 | M | GBGS | 
| 02 | ikawa | EGR-G01 | M | 129 | 
PROFILE/EXAM REQUESTED: IMPACT I PCR Profile
| PCR evaluation for: Ectromelia, EDIM, Hantaan, K virus, LCMV, LDEV, MAD, mCMV, MHV, MNV, MPV, MTV, MVM, Mycoplasma sp., Polyoma, PVM, REO3, Sendai, TMEV GDVII | 
GENERAL COMMENTS: The IDs on the tubes did not match with the submitted paperwork. The ID's on each tube are listed in the table above.
SUMMARY: All test results were negative.
If you have questions, please call our toll free number at 1-800-669-0825 or e-mail us at radil@missouri.edu.
Case Number:  9931-2009
Page  2
PCR EVALUATION:
| Specimen: cells | 01 | 02 | ||||||||
| Ectromelia | - | - | ||||||||
| EDIM | - | - | ||||||||
| Hantaan | - | - | ||||||||
| K virus | - | - | ||||||||
| LCMV | - | - | ||||||||
| LDEV | - | - | ||||||||
| MAD | - | - | ||||||||
| mCMV | - | - | ||||||||
| MHV | - | - | ||||||||
| MNV | - | - | ||||||||
| MPV | - | - | ||||||||
| MTV | - | - | ||||||||
| MVM | - | - | ||||||||
| Mycoplasma sp. | - | - | ||||||||
| Polyoma | - | - | ||||||||
| PVM | - | - | ||||||||
| REO3 | - | - | ||||||||
| Sendai | - | - | ||||||||
| TMEV GDVII | - | - | 
(LEGEND:  + = positive    - = negative    id:id = pooled sample    blank = test not performed
equ = equivocal    NT or blank = no test performed    sus = suspect    wps = weak positive)